A HPLC method for the determination of entrapment efficiency of a new 5-FPE liposome formulation

HPLC法测定一种新型鬼臼毒素衍生物脂质体的包封率(英文)

A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column （350 mm×4.6 mm, 5 μm）. The mobile phase was consisted of methanol-water （58：42, v/v）. The flow rate of mobile phase was set at 0.8 mL/min. The UV detection wavelength was 271 nm, and the column temperature was 30 ℃. The linear range of 5-FPE was from 0.8-12.8 μg/mL, r = 0.9999. The RSD of intm-day and inter-day precision were less than 2.97%. The average recovery was from 96.8%-104.6% with RSD less than 2.24%. The method was simple, rapid, accurate, and sensitive. It is suitable for the determination of entrapment efficiency of the 5-FPE liposome formulation.

建立采用高效液相色谱法测定5-FPE脂质体包封率的方法。色谱柱为Kromasil 100 A C18column(350mm×4.6mm,5μm),流动相为甲醇-水(58:42,v/v),流速为0.8mL/min,测定波长为271nm,进样量为10μL,柱温为30oC。线性范围为0.8～12.8μg/mL,相关系数r=0.9999,日内和日间精密度的RSD小于2.97%,平均回收率为96.8%～104.6%,RSD小于2.24%。本文建立了采用高效液相色谱测定5-FPE脂质体包封率的方法,该方法简便、快速、精确、灵敏度高。