印度萝芙木组织培养技术研究

Study on Rauvolfia serpentina in Vitro

以印度萝芙木枝条顶芽为外植体进行离体培养,外植体最佳灭菌方法为0.08%HgCl2处理时间20min;诱导愈伤组织培养基为MS+L-半胱氨酸0.02mg·L-1+6-BA2.0mg.L-1+NAA0.2mg·L-1,诱导率可达100%;诱导不定芽培养基为MS+L-半胱氨酸0.01mg·L-1+6-BA1.0mg·L-1+NAA0.1mg·L-1;生根培养基为1/2MS+NAA1.0mg·L-1,生根率可达100%。

Taking terminal buds of Rauvolfia serpenfina as materials, the result showed that the optimum disinfection was in 0.08 %Hgcl2 for 20min, 100 percent of callus induction can be reached in medium of MS＋ L-Cysteine 0.02 mg · L-1＋6-BA 2.0mg · L-1 ＋NAA0.2 mg · L-1. The medium of MS＋ L-Cysteine 0.01 mg · L-1＋6-BA 1.0mg · L-1 ＋ NAA 0.1 mg · L-1 was the best one for shoot regeneration from caUi. The optimum medium for root induction was I/2MS＋NAA1.0 mg · L-1 （100%）.