香稻PRO基因的克隆及表达载体的构建

Clonging of PRO Gene from Aromatic Rice and Constructing of Its Expression Vector

利用逆转录-聚合酶链式反应(RT-PCR)得到在水稻中特异性表达的脯氨酸氧化酶(PRO)基因。DNA序列分析表明,所获得水稻的PROcDNA的最大开放阅读框序列全长为1 428 bp,可编码476个氨基酸。该序列与NCBI网站上已发表的PRO基因100%相似。为了能进一步验证所克隆的序列是我们所需的目的基因,成功构建了PRO基因超表达载体和PRO基因干涉载体,便于导入水稻中进行基因的功能鉴定。

By RT-PCR method the cDNA of PRO gene was obtained. Analysis of DNA sequence showed that the largest open reading frame sequence of PRO cDNA was 1 428 bp, which could encode 476. amino acids. The sequence with the NCBI web site has been published by the PRO gene 100% similarity. In order to further verify the sequence by which we cloned the gene required for the successful construction of a over expression PRO gene vector and interference expression PRO gene vector, to facilitate the import of rice carried out to identify the function of genes.