离子注入对凤仙试管苗组织培养和成花的影响

Effects of P^+ ion Implantation on Culture and Flower Formation of Impatiens balsamina in vitro

以茶花凤仙风干种子为材料,研究磷离子注入后对其组织培养的生物学效应。研究表明,注入磷离子各处理的种子萌发率和试管苗株高均高于对照组,且随处理剂量的提高萌发率降低,平均株高增加;处理组试管苗分化率和生根率均低于对照组;低能磷离子注入能够诱导凤仙组培苗试管内开花,处理剂量为1×1016ions/cm2时成花率最高。

The study was carried out by using dry seeds as a treatment material and phosphor ion as an inducing source on Impatiens balsamena offspring. The results showed that the germinant rate, plantlet height of phosphor ion treatment were higher than control in vitro, and with dosage rising the germinant rate reduced, while the plantlet height increased ; the differentiation rate and rooting rate of the treated material were lower than control in vitro ; the P ＋ ion treatment could induce Impatiens balsamena flowering in vitro, and the impregnation with 1 × 10^16 ions/cm^2 was most beneficial to flower.