摘要
利用3种不同来源的柑橘衰退病毒(Citrus tristeza virus,CTV)抗体(PAb-L5、PAb-I、MAbs-S4)对武汉地区发生的CTV进行检测,筛选到一个表现特异血清学性状的分离物CTV-HB1,该分离物仅同来源于中国的多克隆抗体PAb-L5发生血清学反应。为揭示该血清学差异的分子机制,对CTV-HB1和其他几个分离物CP基因序列进行了分析,结果显示:CTV-HB1CP基因与其他19个分离物的核苷酸序列相似性在90.4%-99.4%,但CTV-HB1和CTV-L5的CP基因氨基酸序列间仅存在3个位点的变异,暗示:此3个氨基酸位点可能与CTV-HB1的血清学特性有关。
In this study, a Chinese Citrus tristeza virus(CTV)isolate HB1 showing unusual serological characteristic was identified using three antibodies from different sources ( PAb-LS, PAb-1, MAbs-S4) in Wuhan, Hubei province. CTV- HBI could only be recognized by a polyclonal antibody(PAb-LS)raised against a Chinese isolate L5 in both ELISA and DTBIA. To understand the molecular mechanisms underlying such anomalous serological property, the sequences of CP gene were identified for CTV-HB1 and other isolates. Comparison of the CP gene sequences from CTV-HB1 with those of other 19 isolates published in GenBank revealed that the sequence similarity was 90.4% to 99.4% at nucleotide level. However,only three amino acid residues in the CP gene from CTV-HB1 were different from that of CTV-L5 .All the above results suggested that the three changed position(s)might be correlated to the serological property of CTV-HB1.
出处
《华北农学报》
CSCD
北大核心
2009年第3期212-214,共3页
Acta Agriculturae Boreali-Sinica
基金
国家"863"计划(2006AA10Z434-04)
青岛农业大学高层次人才启动基金(630812)
关键词
柑橘衰退病毒
CP基因
抗体
序列分析
Citrus tristeza virus
CP gene
Antibody
Sequence analysis