MTG酶催化羊毛固定化溶菌酶的酶学性质及抗菌性能

Antibacterial action and properties of lysozyme immobilized on wool catalyzed by MTG

利用新型的生物催化剂微生物谷氨酰胺转胺酶(MTG)催化溶菌酶在羊毛织物上的固定化。采用SDS-PAGE法探讨了溶菌酶作为MTG催化底物的可行性以及固定化溶菌酶的酶学性质及抗菌性能。研究结果表明,羊毛织物经过高锰酸钾预处理后可以实现溶菌酶的固定化。固定化酶的pH值稳定性和温度稳定性优于游离酶;与物理吸附法相比,MTG催化法可赋予固定化溶菌酶的羊毛织物更好的耐水洗性和操作稳定性;此外,固定化酶的低温(4℃)干储藏稳定性良好,30天内酶活仍维持在80%以上。抗菌测试结果表明,MTG催化法固定化溶菌酶的羊毛织物对金黄色葡萄球菌的抑菌率达73.23%,抗菌效果良好。

A new biocatalyst microbial transglutaminase （MTG） was used to immobilize lysozyme on wool fabrics. In this process, sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） was used to verify that MTG can catalyze lysozyme. Besides, the enzymology characteristics and antibacterial performance of immobilized lysozyme was discussed. The result showed that lysozyme could be immobilized on wool pretreated by potassium permanganate. Compared with free lysozyme, the immobilized enzyme showed better stability to pH and temperature. Lysozyme immobilized on wool catalyzed by MTG had better washing and operational stability than that immobilized only by adsorption. In addition, storage stability of immobilized lysozyme was better in dry condition at 4 ℃, and the enzyme activity was over 80% in 30 days. The antibacterial test of the wool immobilized lysozyme showed inhibition percentage of staphylococcus aureus of 73.23%.