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pH梯度萃取-碱性氧化铝柱层析分离长春花中的文多灵和长春质碱 被引量:2

Separation of vindoline and catharanthine from catharanthus roseus by pH gradient extraction and alkaline aluminum oxide column chromatography
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摘要 利用生物碱在不同pH值下存在形式不同的性质,采用pH梯度萃取法将长春花总生物碱分为单吲哚生物碱部分(含有文多灵和长春质碱)和双吲哚生物碱部分(含有长春碱),然后采用碱性氧化铝柱层析对单吲哚生物碱部分进行分离。pH梯度萃取后得到的单吲哚生物碱部分中文多灵和长春质碱的含量分别为18.12%和11.44%,收率分别为80.86%和88.91%;经碱性氧化铝柱层析分离后,得到文多灵和长春质碱含量分别为85.56%和76.73%,收率分别为85.23%和86.34%;重结晶后文多灵和长春质碱纯度分别达到95.22%和98.46%,收率分别为92.15%和98.24%。此方法适合文多灵和长春质碱的工业化大规模生产。 Single indole alkaloids(containing vindoline and catharanthine) and double indole alkaloids (containing vinblastine) were separated from the total alkaloids of Catharanthus roseus by pH gradient extraction according to their different existing statuses under different pH values. The single indole alkaloids part was further isolated by alkaline aluminum oxide column chromatography. After the process of pH gradient extraction, the contents of vindoline and catharanthine are 18.12% and 11.44%, respectively, and their recoveries are 80.86% and 88.91%, respectively. After alkaline aluminum oxide column chromatography, the contents of vindoline and catharanthine are 85.56% and 76.73%, respectively, and their recoveries are 85.23 % and 86.34%, respectively. After recrystallization, the contents of vindoline and catharanthine are 95.22% and 98.46%, and their recoveries are 92.15% and 98.24%, respectively. This process is suitable for large-scale industrial production of vindoline and catharanthine.
出处 《化工进展》 EI CAS CSCD 北大核心 2009年第7期1282-1286,共5页 Chemical Industry and Engineering Progress
基金 国家"十一五"科技支撑项目(2006BAD18B0401) 东北林业大学青年科研基金项目(07041)
关键词 pH梯度萃取 碱性氧化铝柱层析 长春花 文多灵 长春质碱 pH gradient extraction alkaline aluminum oxide column chromatography catharanthus roseus vindoline catharanthine
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参考文献11

  • 1Whitmer S, Canel C, Hallard D, et al. Influence of precursor availability on alkaloid accumulation by transgenic cell line of catharanthusroseus[J].Plant Physiol., 1998, 11(6): 853-857.
  • 2Van D H R, Jacobs Di, Snoeijer W, et al. The Catharanthus alkaloids: pharmacognosy and biotechnology[J].Current Medicinal Chemistry, 2004, 11(5): 607-628.
  • 3Kutney J P, Boulct C A, Choi L S Lct al. Alkaloid production in Catharanthus roseus (L.) G. Don cell cultures. XV. Synthesis of bisindolc alkaloids by use of immobilized enzyme systems[J]. Heterocycle, 1988, 27: 621-628.
  • 4Jordan M A, Thrower D, Wilson L. Mechanism of inhibition of cell proliferation by Vinca alkaloids[J]. Cancer Res., 1991, 51(8): 2212-2222.
  • 5Zhou X J, Rabmani R. Preclinical and clinical pharmacology of vinea alkaloids[J]. Drugs, 1992, 44(Suppl 4): 1-16.
  • 6Ngan V K, Bellman K, Hill B T, et al. Mechanism of mitotic block and inhibition of cell proliferation by the semi-synthetic Vinca alkaloids vinorelbine and its newer derivative vinflunine[J]. Mol. Pharmac., 2001, 60(1): 225-232.
  • 7Okouneva T, Hill B T, Wilson L, et al. The effects of vinflunine, vinorelbine, and vinblastine on centromere dynamics[J]. Mol. Cancer Ther., 2003, 2(3): 427-436.
  • 8Renault J H, Nuzillard J M, Le C G, et al. Isolation of indole alkaloids from catharanthus roseus by centrifugal partition chromatography in the pH-zone refining modep[J]. J. Chromatogr. A, 1999, 849(2): 421-431.
  • 9张琳,杨磊,牛卉颖,祖元刚.大孔树脂吸附分离长春花中的文多灵、长春质碱和长春碱[J].化工学报,2008,59(3):607-614. 被引量:18
  • 10罗猛,付玉杰,祖元刚,史权,牟璠松,李庆勇.反相高效液相色谱法快速测定长春花中4种生物碱[J].分析化学,2005,33(1):87-89. 被引量:50

二级参考文献45

  • 1罗猛,付玉杰,祖元刚,史权,牟璠松,李庆勇.反相高效液相色谱法快速测定长春花中4种生物碱[J].分析化学,2005,33(1):87-89. 被引量:50
  • 2林强,霍清.大孔吸附树脂对苦参碱的吸附分离研究[J].北京联合大学学报,2005,19(4):80-82. 被引量:5
  • 3Szuhaj B F.Lecithins:Sources,Manufacture & Uses.Champaign:AOCS Press,1989.109-130.
  • 4Juneja L R, Sugino H, Fujiki M, Kim M,Yamamoto T.Preaparation of pure phospholipids from egg yolk.In: Sim J S, Nakai S,Guenter W, eds. Egg Nutrition and Biotechnology.UK: CABI Publishing, 2000.139-149.
  • 5徐小红.[D].Hangzhou: Zhejiang University,2002.
  • 6Stephen H,Stephen T.Solubilities of inorganic and organic compounds,VolumeⅠ,PartⅠ.New York: Pergamon Press,1963. 370.
  • 7Volkov S K, Grodnitskaya E I. J. Chromatogr. B, 1994, 660(2): 405-408
  • 8Editorial Committee of the Pharmacopocia of the Pelple′s Republic of China(中华人民共和国药典委员会编). The Pharmacopocia of the Pelple′s Republic of China, vol 2(中华人民共和国药典(二部)). Beijing(北京): Chemical Industry Press(化学工业出版社), 2000: 854-856
  • 9Tikhomiroff C, Jolicoeur M. J. Chromatogr. A, 2002, 955(2): 87-93
  • 10Pennanen S, Huhtikangas A. Photochem. and Photobiology, 1990, 51(5): 515-518

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