Expression Profile Changes of Genes Involved in Lipid Metabolism Pathway During Liver Regeneration in Mice

小鼠肝再生过程中脂质代谢相关通路中基因的表达谱变化(英文)

[ Objective ] The aim of the research was to study the expression profile changes of genes involved in lipid metabolism pathway during liver regeneration in mice. [ Method] The CCI4 induced mouse model of liver regeneration was established and the total RNA was isolated from liver tissue of mouse. Then the changes of genes involved in lipid metabolism pathway during different stages of liver regeneration were detected through micro-array chip gene technique and their specific functions were also analyzed. [ Result] Dudng the process of liver regeneration, the expression level of 98 genes involved in lipid metabolism pathway changed, which were divided into eight groups according to change trend. In the mass, the expression of genes was inhibited in the early stage and up-regulated in the late phase. And the gene expression associated with fatty acid synthesis pathway was mainly up-regulated while the catabolic pathway did not change significantly. Most of genes involved in bile acid synthesis pathway were suppressed before 4.5 d and up-regulated after 4.5 d or 7 d. [ Conclusion] During the process of liver regeneration, the genes associated with lipid metabolism are expressed in different trends, and this data should provide a specific range of genes for further studying the regulation effect of lipid metabolism related pathway on liver regeneration.

1材料与方法1．1材料 试验动物：9周龄的雄性C57BIV6小鼠，自由采食和饮水，每天光照12h，黑暗12h。试剂：TRIzol试剂（Invitrogen）；RNeasy Total RNA Mini Kit（Qiagen）；基因芯片Genechip mouse genome 43020（Affymetrix）；17启动子引物序列（Affymetrix）。