哮喘患者诱导痰炎性细胞中Tim-3 mRNA表达的研究

Tim-3 mRNA Expression in Induced Sputum from Asthmatic Patients

目的检测哮喘患者诱导痰炎性细胞中Tim-3 mRNA的表达,并分析其与上清液中IL-4、IFN-γ的水平及肺功能的相关性,探讨Tim-3 mRNA在哮喘发生发展中的作用。方法2007年10月至2008年5月在华中科技大学同济医院哮喘门诊或住院患者中收集哮喘缓解期患者25例,轻至中度急性发作期患者28例,利用逆转录-聚合酶链反应技术(RT-PCR)检测哮喘患者诱导痰炎性细胞中Tim-3 mRNA的表达并做半定量分析,利用酶联免疫吸附试验(ELISA)检测上清液中IL-4、IFN-γ的水平,并测定所有患者的肺功能,分析Tim-3 mRNA与IL-4、IFN-γ的水平及肺功能的相关性。结果哮喘发作期诱导痰炎性细胞中Tim-3 mRNA吸光度值为(0.48±0.07),与缓解期的(0.21±0.04)和对照组(0.09±0.03)比较,差异均有统计学意义(均P<0.05),缓解期与对照组比较差异有统计学意义(P<0.05);哮喘发作期诱导痰上清液中IL-4的水平为(39.71±8.37)pg/ml,与缓解期的(21.63±9.18)pg/ml,和对照组(16.24±7.56)pg/ml比较,差异均有统计学意义(均P<0.05),缓解期与对照组比较差异有统计学意义(P<0.05);哮喘发作期诱导痰上清液中IFN-γ的水平为(32.64±6.45)pg/ml,与缓解期的(78.51±8.62)pg/ml和对照组(86.47±9.31)pg/ml比较,差异均有统计学意义(均P<0.05),缓解期与对照组比较差异有统计学意义(P<0.05)。哮喘发作组诱导痰炎性细胞中Tim-3 mRNA的表达水平与IL-4呈正相关(r=0.65,P<0.05),与IFN-γ和肺功能分别呈负相关(r=-0.89,r=-0.78,均P<0.01)。结论诱导痰炎性细胞中Tim-3 mRNA表达增高可能参与哮喘的发生与发展。

Objective To detect the expression of Thl-specific cell surface protein T cell immunoglobulin mucin 3 （Tim-3） mRNA in induced sputum isolated from asthmatic patients, analyze its correlation with the level of interleukin 4 （IL-4）, interferon-7 （IFN-7） and pulmonary ventilatory capacity, and explore the role of Tim-3 in the occurrence and development of asthmatic inflammation. Methods From October 2007 to May 2008, 28 patients with acute asthma and 25 patients with asthma in remission stage were collected from Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. The Tim-3 mRNA expression was detected by using reverse transcription-polymerase chain reaction （RT-PCR） method. The IL-4 and IFN-γ levels were determined by ELISA. The correlation among Tim-3 mRNA, IL-4, IFN-7 level and pulmonary ventilatory capacity was analyzed. Results The expression of Tim-3 mRNA in patients with acute asthma was （0. 48±0.07）, significantly higher than that in those with asthma at remission stage and controls [ （0.21 ±0.04） and （0.09 ± 0.03） respectively, both P〈0.05]. The expression of Tim-3 mRNA in patients with asthma in remission stage was significantly higher than that in controls （P〈0.05）. The level of IL-4 in patients with acute asthma was （39.71±8.37）pg/ml, significantly higher than that in those with asthma in remission stage and controls （21.63±9.18） and （16.24±7.56）pg/ml respectively （both P 〈0.05）. The level of IL-4 in patients with asthma in remission stage was significantly higher than that in controls （P〈0.05）. The level of IFN-γ in patients with acute asthma was （32.64±6.45）pg/ml, significantly lower than that in those with asthma in remission stage and controls [（78.51±8.62） and （86.47±9.31）pg/ml respectively, P〈0. 05]. The level of IFN-γ in patients with asthma in remission stage was significantly lower than that in controls （P〈0.05）. The Tim-3 mRNA expression was positively correlated with the level of IL-4 （r=0. 65, P〈0.05）, and negatively with the level of IFN-γ and pulmonary ventilatory capacity （r=- 0.89, r=- 0.78, P〈0. 01）. Conclusion The increased expression of Tim-3 mRNA in induced sputum might take part in the occurrence and development of asthmatic inflammation.