复合膜引导牙周组织再生

Complex Membrane Guided Periodontal Tissue Regeneration

目的:观察基因重组人骨形成蛋白-2、胶原、聚乳酸/聚羟基乙酸共聚体(rhBMP-2/Co/PLGA)复合膜引导牙周组织再生的效果。方法:在杂犬的第四前磨牙及第一磨牙颊侧部位,制备急性牙周组织缺损模型,将复合膜材料植入实验部位,设对侧为同体对照。术后8周处死动物,切取双侧下颌骨标本,行软X线照相和组织学观察。结果:实验侧可见大量新生牙槽骨、牙骨质和牙周膜纤维形成;对照侧主要是牙龈上皮和上皮下结缔组织增生。结论:rhBMP-2/Co/PLGA复合膜符合GTR的要求,能够引导牙周组织再生。

Objective： To observe the effect of rhBMP- 2/collagen/PLGA complex membrane in periodontal tissue regeneration, and evaluate the membrane function for GTR. Methods： The fourth premolars and the first premolars of 7 healthy adult hybrid dogs were used in the study. The right sides were experiment sides, rhBMP -2/collagen/PLGA com- plex membrane were placed and no membranes in the left sides （ the control areas）. The animals were sacrificed after 8 weeks. Mandibulars samples were removed, examined by eye and soft X - Ray, decalcified in EDTA, embedded, sec- tioned, HE stained and followed by light microscope examination. Results： In the experiment sides, new alveolar bone, cementum and periodontal ligament fiber were formed, however gingival epithelium proliferated and connected tissue were formed in the control sides. Conclusion： rhBMP- 2/collagen/PLGA complex biomembrane can be used as a material for GTR application.