BDNF对端侧吻合面神经再生作用的研究

Effects of BDNF on Peripheral Facial Nerve Regeneration after End-to-side Neurorrhaphy

目的:探讨脑源性神经营养因子(BDNF)对面神经端侧吻合后促进神经再生的作用。方法:30只家兔随机分2组,将双侧离断的面神经上颊支远端与同侧外膜开窗的下颊支行端侧吻合。术后给予BDNF的为实验组,给予生理盐水的为对照组。分别于术后2、4、8周取材,采用电生理,光镜,透射电镜等检测方法。结果:实验组神经传导速度、动作电位、组织学形态学定量分析等指标明显优于对照组。结论:BDNF对面神经端侧吻合后神经再生有明显促进作用。

Objective： To investigate the effect of brain - derived neurophin fat （BDNF） on peripheral facial nerve re- generation after end - to - side neurorrhaphy. Methods ： 30 rabbits were randomly divided into two groups. The distal end of the upper - cheek branch of facial nerve was transected to the down - check branch with epineurial window in an end - to - side fashion. BDNF was injected into the experimental group side postoperatively. The contral group was injected with 0.9% saline（NS）. Postoperative evaluation was performed by electrophysiological test and morphological observation after 2,4,8 weeks. Results： The experimental group~ nerve conduction velocity, action potential and the histopathology quantity analysis were significant superior than the contral group. Conclusin： BDNF can significant promote regeneration of facial nerve after end - to - side neurorrhaphy.