17β-雌二醇对新生牛血清诱导的血管平滑肌细胞增殖反应的影响及其机制

Influence of 17β-estradiol on Vascular Smooth Muscle Cells Proliferation Induced by Fetal Calf Serum and Its Mechanism

目的:探讨雌甾-1,3,5(10)-三烯-3,17β-二醇(17β-雌二醇)对新生牛血清诱导血管平滑肌细胞(VSMCs)增殖反应的影响及其机制。方法:用新生牛血清刺激培养的VSMCs增殖,MTT法检测17β-雌二醇对新生牛血清诱导的VSMCs增殖的影响,并用Western blot检测caveolin-1蛋白,诱导型一氧化氮合酶(iNOS)及NO在此过程中的变化。结果:与对照组比,新生牛血清可促进VSMCs增殖(P<0.05),17β-雌二醇作用24 h后能明显抑制上述作用(P<0.05);17β-雌二醇预处理可抑制新生牛血清诱导的VSMCs中caveolin-1,iNOS蛋白表达下降及NO释放(P<0.05);17β-雌二醇受体阻断剂1-{4-[2-(n,n-二甲氨基)乙氧基]苯}-1,2-二苯-1-丁烯(他莫昔芬)预处理可部分逆转17β-雌二醇的抑制作用(P<0.05)。结论:17β-雌二醇通过其受体可抑制新生牛血清诱导的VSMCs增殖,此过程与上调caveolin-1蛋白表达,激活iNOS,增加NO释放有关。

Objective： To investigate the influence of 17β-estradiol on vascular smooth muscle cells（VSMCs）proliferation induced by fetal calf serum and the related mechanism. Methods： The fetal calf serum was used to stimulate cultured VSMCs preliferation（ P 〈0.05）, and the effects of estrogen on VSMCs proliferation were determined by MTr, and the expressions of caveolin-1 and inducible nitric oxide synthase（iNOS）were also detected by Western blot. Results： Compared with the normal control group, the fetal calf serum could increase the VSMCs proliferation（ P 〈 0.05）, and inhibit proliferation effect of fetal calf serum, pretreated with estradiol for 24 hours （ P 〈 0.05）. Western bolt results further proved that 17β-estradiol inhibited the downregulation of caveolin-1, iNOS and NO induced by fetal calf sermn, which was prevented by pretreatment with estrogen antagonist, Tamoxifen（ P 〈 0.05）. Conclusion： 17β-estradiol can inhibit VSMCs proliferation induced by fetal calf serum, and this effect is mediated by estrogen receptor. 17β-estradiol increases caveolin-1 and iNOS protein expression, and NO release, leading to the inhibition of VSMCs proliferation induced by fetal calf serum.