增殖细胞核抗原和细胞周期依赖性激酶抑制剂p21WAF1/CIP1蛋白在大鼠肾上腺细胞体外原代培养中的表达及其意义

Expression and Significance of Proliferating Cell Nuclear Antigen and p21WAF1/CIP1 in Rats Adrenocortical Cell Culture in vitro

目的:探讨增殖细胞核抗原(PCNA)和细胞周期依赖性激酶抑制剂p21 WAF1/CIP1蛋白在大鼠肾上腺细胞体外原代培养中的表达及其意义。方法:用SP免疫组化法检测大鼠肾上腺细胞培养第3、5、7、9、11 d的PCNA和p21蛋白表达强度。结果:在肾上腺细胞体外培养中,第3 d((18±3)%)、5 d((38±5)%)、7 d((70±65)%)PCNA蛋白表达逐渐增强,第9((50±3)%)、11 d((39±2)%)与第7 d比减弱(P<0.05)。第3 d((43±5)%)、5 d((35±2)%)、7 d((21±3)%)p21 WAF1/CIP1蛋白表达逐渐减弱,第9 d((39±3)%)、11 d((52±4)%)与第7 d比重新增强(P<0.05)。结论:肾上腺细胞体外原代培养在第7 d PCNA蛋白表达最强,p21 WAF1/CIP1蛋白表达最弱,肾上腺细胞增殖也最旺盛。

Objective： To explore expression and significance of proliferating cell nuclear antigen（PCNA）and cyclin dependent kinase inhibitor p21WAF1/CIP1 in rots adrenocortical cell culture in vitro. Methods： Immunohistochemical method was adopted to examine the expressions of PCNA and p21WAFI/CIP1 in cell culture at d3, d5, d7, d9 and dll. Results： In adrenoeortical cell culture /n vitro, the expression of PCNA was gradually enhanced at d3（（18 ± 3）%）, d5（（38 ± 5）%）and d7（（70 ± 65）%）, but were gradually decreased at d9（（50 ± 3） %）and d11（（39 ± 2）%） （ P 〈 0.05）, and the expression of p21WAF1/CIP1 was decreased at d3（ （43 ± 5） % ）, d5（ （35 ± 2） % ）and 7d（ （21 ± 3）%）, but were gradually enhanced at d9（（39 ± 3）%）or d11（（52±4）%）（P 〈0.05）. Conclusion： The proliferation of adrenoeortical cells is the most vigorous in vitro at d7.