HPLC法测定近视明眼用凝胶中异紫堇定的含量

HPLC Determination of Isocorydine in JinshiMing Eye Gelatin

目的:建立近视明眼用凝胶中异紫堇定的含量测定方法。方法:用AgilentTC-C18色谱柱(4.6 mm×150 mm,5μm),以甲醇-0.2%磷酸水溶液(用三乙胺调pH值7.0)(54∶46)为流动相,检测波长为267 nm,流速1.0 mL.min-1。结果:异紫堇定进样量在0.123 0～1.107 0μg范围内线性关系良好,平均回收率为97.11%,RSD为1.65%。结论:该方法简便、准确、重复性好,可用于近视明眼用凝胶的质量控制。

Objective：To develop an HPLC method for determination of Isocorydine in Jinshining Eye gelatin. Method： AgiLentTC-C18 chromatographi coLumn（4.6 mm× 150 mm,5 μm） was used,methanoL-0.2% phosphoric acid soLution （adjust the pH 7.0 take methyL aLcohoL）（54：46）as the mobile phase, the detection waveLength was 267 nm, fLow rate at 1.0 mL· min^-1 . Result： The calibration curve of Isoeorydine as linear between 0.123 0 - 1.107 0 μg, The average recovery and the relative standard deviation were 97.11% and 1.65 %. Conclusions：The method is simple, accurate, repeatable. It can be used for quality control of JinshiLe Eye gelatin.