黄芪对缺血性急性肾功能衰竭的保护作用

Protective Effect of Astragalus Membranaceus on Ischemic Acute Renal Failure

目的:观察黄芪对缺血性急性肾功能衰竭(ARF)时血清肝细胞生长因子(HGF)水平、肾功能及肾组织病理学改变的影响,探讨黄芪肾脏保护作用的机制。方法:通过切除大鼠右肾,夹闭左肾动脉60 min后再灌注(I/R),建立IARF模型。治疗组术前3 d连续给予黄芪ip,其他组(假手术组及I/R 0、4、12 h组)应用生理盐水。测量各组血清HGF、尿素氮(BUN)、肌酐(Cr)水平,并通过光镜下肾小管评分来评价肾组织病理学改变。结果:I/R 4 h对照组血清HGF水平与假手术组无显著差异(P>0.05);黄芪组血清HGF水平较I/R 4 h对照组明显升高(P<0.01);I/R对照组血清BUN、Cr水平较假手术组明显升高(P<0.01),黄芪组可以减少血清BUN、Cr水平升高的程度(P<0.05);同时也可以使肾组织病理损伤显著改善(P<0.05)。结论:在IARF模型,黄芪预处理可以减轻肾脏损伤,改善肾功能。黄芪诱导HGF生成增加可能是其肾脏保护作用的机制之一。

Objective： To investigate the effect of astragalus on the serum levels of hepatocyte growth factor （HGF）, renal function and the damage of renal morphology in ischemic acute renal failure （IARF）. To study the protective mechanism of astragalus in IARF. Methods： The rats were pretreated with astragalus or vehicle for 3 days. IARF was induced by left renal artery clipping for 60 rain, after the right kidney was removed. Biochemical parameters for serum HGF, blood urea nitrogen（BUN）, creatinine（Cr） were measured and histological analyses of the kidneys were performed by tubular grading. Results： There have no significant difference between the serum HGF levels of I/R controls and the levels observed in sham-operated group （ P 〉 0.05 ）. Serum HGF levels in astragalus treated group was significantly increased than those of I/R control group（ P 〈 0.01 ）. After 4 h of I/R, serum levels of BUN and Cr were increased in I/ R control rats compare with Sham-operated rats （ P 〈 0. 01 ）. Astragalus treatment reduced the levels elevation （ P 〈 0.05）. Simultaneously, I/R-induced severe damage in renal morphology were significantly ameliorated by astragalus treatment（ P 〈 0.01 ）. Conclusions： Astragalus pretreatment can alleviate the damage of renal function in IARF. It is may be one of the mechanisms of astragalus for protecting renal tissue in IARF that astragalus can induce the expression of HGF.