冠心病血瘀证与肌动蛋白相关基因异常表达的相关性

Correlation between Blood Stasis Syndrome of the Coronary Heart Disease and the Abnormal Expression of Actin-related Genes

目的探讨冠心病血瘀证与肌动蛋白相关基因异常表达的相关性。方法依据病证结合的研究思路,将5例患者样本混合与5名正常人样本混合并杂交制备芯片(即病证结合芯片)。根据治疗前后血液黏度测试结果设计瓜蒌薤白半夏汤治疗前后自身对照芯片(以下称"以药测证芯片"),并对差异表达基因进行信号通路分析,筛选出凝血、血液流变学相关差异表达基因及其与肌动蛋白细胞骨架调控相关的通路。结果在两张芯片中共同表达的凝血、血流变功能方面差异表达的基因有肌动蛋白(ACTA2)、纤维连接蛋白(FN1)、结合珠蛋白(Hp)、骨桥蛋白(SPP)。其与细胞通讯、黏着斑、细胞外基质-受体相互作用、肌动蛋白细胞骨架调控、转化生长因子-β信号通路(TGF-β)等5条通路有关。结论冠心病血瘀证与肌动蛋白基因及其代谢调控相关,介导了凝血、血流变异常基因的5条信号转导通路。

Objective To study the correlation between the blood stasis syndrome of the coronary heart disease （CHD） and the abnormal expression of actin-related genes. Methods Based on the study of disease-syndrome combination, the 5 sample cases and the 5 normal samples were mixed respectively, and the disease-syndrome chips were made from hybridization of the mixed samples. The self control chips of Gualou Xiebai Banxia Decoction were designed according to the blood viscosity before and after treatment. The signal pathway was analyzed on the different expression genes to find out the hemoglutination and blood rheology related expression genes and the pathway of the regulation of actin cytoskeleton. Results In the two chips, ACTA2, fibroneetin （FN1）, haptoglobin （Hp）, and secreted phosphoprotein （SPP） were those genes which had different expressions of hemoglutination and blood rheology. They were related with the five pathways, including the cell communication, focal adhesion, ECM-receptor interaction, regulation of actin cytoskeleton, and TGF-beta signaling pathway. Conclusion The blood stasis syndrome of CHD is correlated with actin gene and its metabolism regulation, introducing the 5 signal transmission pathways of abnormal genes of hemoglutination and blood rheology.