左归丸对去势雌性大鼠子宫和阴道的影响

Effect of Zuogui Pill on the Uterus and Vagina of Ovariectomized Female Rats

目的观察左归丸对去势大鼠子宫和阴道的影响。方法将雌性育龄期大鼠分为正常组(20只)、假手术组(17只)和去势组(40只)。去势组将去势成功的大鼠分为模型组(8只)、倍美力组(11只)、左归丸高剂量组(左高组,6只)、左归丸低剂量组(左低组,9只),药物连续灌胃11周后,测定血清雌二醇(E2),取子宫、阴道组织作病理切片。结果与模型组比较,左高组、左低组大鼠子宫的重量、脏器指数、内膜厚度、内膜被覆上皮厚度、肌层厚度、腺体直径、内径、腺上皮高度、腺体个数和阴道的重量、脏器指数、上皮层数和黏膜厚度差异均无统计学意义(P>0.05);左高组阴道皱襞数和阴道固有层血管数均高于模型组(P<0.05);血清E2值左高组、左低组差异无统计学意义(P>0.05)。结论补肾中药复方左归丸对去势大鼠子宫无明显影响,可通过增加局部血液供给来延缓去势大鼠的阴道衰萎。

Objective To observe the effect Zuogui Pill （Yin-restoring Pill） on the uterus and vagina of ovariectomized female rats. Methods The female rats at childbearing age were randomized into normal group in= 20）, sham-operation group in= 17）, and ovariectomized group in= 40）. After ovariectomy, the ovariectomized group was subdivided into model group in= 8）, Premarin group in=11）, Zuogui Pill large dosage group in=6）, and Zuogui Pill small dosage group in= 9）. The medication was given through gastric perfusion for 11 weeks in succession. The serum estradiol （E2） was tested and the uterine and vaginal tissues were examined with the method of pathological section. Results Compared with the model group, there were no statistical difference in the uterine weight, viscera index, endometrial thickness, efldometrial epithelial thickness, muscular thickness, gland diameter, inner diameter, gland epithelial height, and number of gland, and the vaginal weight, viscera index, epithelial layer number and mucosal thickness in the Zuogui Pill large and small dosage groups （P〉0. 05）. The number of vaginal folds and vessle of vaginal natural layers in the Zuogui Pill large dosage group were more than those in the model group （P〈0. 05）. Serum E2 in Zuogui Pill large dosage group was not significantly different from that in the Zuogui Pill small dosage group （P〉0. 05）. Conclusion Zuogui Pill, the compound herbal formula for reinforcing the function of kidney, is not influential on the uterus of ovariectomized female rats. It can delay the vaginal deadness through increasing the local blood supply of ovariectomized rats.