氨基酸对烷烃降解菌GS3C降解性能的影响

Effect of Amino Acids on Degrading Capability of a n-alkanes Degrading Strain GS3C

从广州石油化工总厂污水处理站旁的油泥混合物中分离筛选得到一株烷烃降解菌GS3C,从生理生化及16S rDNA基因扩增测序等方面进行了鉴定,并在振荡好氧培养条件下研究该菌的降解性能及酵母浸膏、碳源、氮源、维生素、水解酪蛋白等营养物对其降解的影响,同时考察不同氨基酸对降解的影响.结果表明,该菌的16S rDNA基因扩增序列与Burkholderia cepacia的同源性为97%,初步断定该菌株属于洋葱伯克霍尔德氏菌(Burkholderia cepacia),酵母浸膏能促进其对正十六烷的降解,其主要因素为氨基酸对降解的促进作用.谷氨酸、脯氨酸、赖氨酸、缬氨酸及亮氨酸的混合物对降解菌GS3C的代谢促进作用最好,其中赖氨酸与脯氨酸为关键的2种氨基酸.

A bacterial strain named GS3C isolated from petroleum-contaminated soil in Guangzhou petrochemical industry was identified based on its morphology, physiological-biochemical characteristics as well as partial 16S rDNA sequence analysis. The effects of yeast extract, carbon source, nitrogen source, vitamins and casein acid on the biodegradation potential were investigated under vibrational and aerobiotic conditions. The effects of each amino acid on the biodegradation potential were also examined. The strain GS3C was identified to be Burkholderia cepacia, since it exhibited 97% similarity in partial 16S rDNA sequence. The results showed that the biodegradation potential to cetane was promoted significantly with the addition of yeast extract. Amino acids were the primary factor for the biodegradation rate, while nutrient carbon sources, nitrogen sources and vitamins were not significant. Further studies also indicated that the combination of L-glutamic acid, L-proline, L-lysine, L-valine and L-leucine were most effective for promoting the biodegradation potential, and L-lysine and L-proline were the two most important amino acids in combination. None of the single amino acid additions were more effective compared with the mixtures.