硫酸软骨素对大鼠心肌膜微血管内皮细胞分泌IFN-γ的影响

Effects of Chondroitin Sulfate on IFN-γ Release of Rat Myocardial Microvascular Endothelial Cells in vitro

研究硫酸软骨素(chondroitin sulfate,CS)对微血管内皮细胞(MVECs)分泌IFN-γ的影响,为进一步研究CS的免疫增强机制提供资料。体外培养大鼠心肌膜微血管内皮细胞(RMMVECs),CS分为10、50、500μg/mL 3个浓度组刺激RM-MVECs,ELISA法检测药物处理后细胞上清液中IFN-γ的含量。结果显示,RMMVECs正常情况下能够分泌一定量的IFN-γ,经CS处理后IFN-γ的分泌量显著升高,且3个剂量组在刺激后24 h分泌量均达到峰值。结果表明,CS诱导大鼠心肌膜微血管内皮细胞分泌IFN-γ可能是其免疫增强作用机制之一。

To study whether chondroitin sulfate （CS） could induce rat myocardial microvascular endothelial cells （RM- MVECs） in vitro to secrete IFN-γ. Using double antibody sandwich ELISA to detect the level of IFN-γ produced by RM- MVECs stimulated with CS in cell supernatant liquid. The level of IFN-γ produced by RMMVECs stimulated with CS in cell su- pernatant liquid was increased dramatically, and got the maximum at 24 h. CS could induce rat microvascular endothelial cells to secrete IFN-γ.