MKR转基因小鼠生物学特性的初步探讨

Preliminary Study of Biological Property of MKR Mice

试验旨在测定MKR转基因2型糖尿病小鼠生物学特性数据,为2型糖尿病研究提供技术平台。由美国国立卫生研究院(NIH)授权使用引进的MKR鼠,经自然交配后繁殖的子代作为研究对象,测定MKR鼠的体重、血糖、记忆力、抗疲劳、耐缺氧、麻醉耐受试验,同期采用昆明小鼠和C57BL/6J小鼠作为对照。结果表明,新生MKR鼠自出生3周开始,血糖增高,体重随年龄的增加而减慢,与对照组小鼠同期比较,具有非常显著性意义,MKR鼠抗疲劳、耐缺氧、麻醉耐受能力比昆明小鼠差(P<0.01)。因此,MKR鼠是一种良好的2型糖尿病的动物模型。

To develop a diabetic mouse model to determine the data of biological property in MKR mice, a model in which expression of a dominant-negative IGF-I receptor in the skeletal muscle leads to systemic insulin resistance and diabetes, so as to provide a platform for researches about the type 2 diabetes. MKR mice were received from the National Institutes of Health, USA, and propagated naturally. These mice were followed up for body weight, blood glucose level, faculty of memory, fatigue- resistant ,oxygen-deficiency-enduring and experiment of anaesthesia tolerance, using wild-type Kunming mice or C57BL/6J mice as controls. Results showed that these mice were hyperglycemia from 3 weeks of age onward and the body weight increase slowed down with increasing of ages, which is very significant than the controlling littermates（P^0.01）. MKR mice in the other were worse than Kunming littermates, that is also significant（P〈0.01）. Therefore, MKR mice model should be estabol- ished as a good king of animal model in the type 2 diabetes.