肾静脉套管法建立大鼠腹部心脏移植模型

An Effective Heterotopic Heart Transplant Model in Rats

目的建立一种快速、有效的大鼠异位心脏移植模型。方法以雄性SD大鼠为供体,Wistar大鼠为受体,将供心的升主动脉与受体的腹主动脉行端侧吻合,切除受体的左肾,将供心的肺动脉与受体的左肾静脉以自制聚乙烯管直接插入法连接。结果35例实验成功32例,成功率91.4%,受体存活时间平均为(25.3±9.8)d。失败的原因:吻合口出血,血栓形成。结论本术式操作简单实用,不需手术显微镜,易于推广。

Objective To establish a quick and effective heterotopic heart transplant model in rats. Method Male SD rats served as donors and Wistar as recipients. The ascending aorta of allograft was anastomosed to the recipient abdominal aorta. The recipient underwent a left nephrectomy, and the right pulmonary artery of allograft was inserted into the left renal vein of donors. Results The mean chemic time was （37.8 ± 1.8） min in 35 experiments. The successful rate was 91.4 % . Complications were anastomotic orificces hemorrhange, and thrombosis. Conclusion This method has advantage of simpleness, and requires no operating microscope. So it is easily to be spread.