2007年重庆市人群沙门菌监测分析

Surveillance of Salmonella in population in Chongqing,2007

目的对重庆市人群沙门菌感染进行监测并对监测分离的菌株进行耐药性和脉冲场凝胶电泳(PFGE)分子分型研究。方法明确监测病例定义,制定、实施监测方案,实验室分离、鉴定监测的沙门菌,利用K-B法对分离菌株进行药敏检测,并采用PFGE进行分子分型。结果2007年监测了835例病例,病例分布在9个区(县),其中监测沙门菌暴发疫情10起,病例175例。实验室共分离鉴定了79株12种沙门菌(11种血清型和1组B群),全部菌株对至少一种抗菌素耐药,9种(75%)对3种以上的抗菌素耐药,2种(16.67%)对6种抗菌素耐药,尤其是里定沙门菌、婴儿沙门菌及汤卜逊沙门菌耐药严重。沙门菌PFGE分型被分成9种14种亚型。结论重庆市2007年人群沙门菌分离株没有明显的优势流行株,同一事件中的沙门菌PFGE分型相同,分离株耐药情况严重,分离菌株的耐药谱和PFGE型也没有明显的联系。

Objective To conduct surveillance of Salmonella in population and study of drug resistance and PFGE typing of Salmonella strains isolated in Chongqing. Methods The surveillance case definition was determined, the surveillance schema was developed and implemented, Salmonella strains were isolated and identified. The drug susceptibility test of isolated Salmonella strains was conducted by Kirby-Bauer method, and the molecule typing was performed by PFGE. Results A total of 835 cases of Salmonella infection were under surveillance in 2007, these cases distributed in 9 districts/counties. Ten Salmonella infection outbreaks were detected （ 175 cases）. Seventy nine strains of Salmonella were isolated, which belonged to 11 serotypes and B serogroup. All the strains were resistant to one antibiotics at least, 9 strains （75%） were resistant to t〉 3 antibiotics, 2 strains （16. 67% ） were resistant to 6 antibiotics. Particularly, Salmonella reading, Salmonella infantis, Salmonella thompson were highly resistant to antibiotics. The Salmonella strains isolated were divided into 9 PFGE types with 14 subtypes. Condusion No predominating strains were detected among the isolates of Salmonella in population in Chongqing in 2007; the PFGE types were same for the strains in an outbreak; the drug resistances of the strains isolated were high and the drug resistance spectrum and PFGE type of isolated strains did not related with each other obviously.