西藏野生油菜遗传多样性分析

Genetic diversity of Tibetan wild rapeseeds detected by AFLPs and SSRs

利用10对AFLP引物及13对SSR引物,分析了43份来自西藏地区部分野生类型油菜种质的遗传多样性。10对AFLP引物共得到276条清晰的谱带,其中多态性带214条,多态性位点比率为77.5%,平均每对AFLP引物得到21.4条多态性带。13对SSR引物共扩增出57条带,其中多态性带51条,多态性位点比率为89.5%,说明西藏野生油菜遗传多样性丰富。通过对西藏野生类型油菜资源的遗传距离以及聚类分析,可将西藏野生类油菜分为两大类群,分别为白菜型和芥菜型野生油菜种质资源;两种分子标记适合揭示西藏野生油菜的遗传多样性。

10 AFLP and 13 SSR primer combinations were used to amplify the 43 wild type rapeseed materials from Tibet. A total of 276 clear bands were produced by 10 AFLP pairs of primers, of which 214 bands were poly-morphism with an average of 21.4 polymorphic bands per AFLP primer pair, and the polymorphic ratio was 77.5%. Meanwhile, 13 SSR primer pairs amplified 57 bands, with 51 polymorphisms, and the polymorphic ratio was 89.5 %. Genetic distance based on AFLP and SSR showed that Tibetan wild type rapeseed germplasm has high genetic diversity. Clustering of most of 43 accessions were coincided with B. campestris or B. juncea, indicating that AFLPs and SSRs are fit for detecting the genetic diversity of Tibet wild rapeseed germplasm. All the results are beneficial for further identification and classification of the rich genetic resources of wild type rapeseed, and for breeding fine new varieties of rapeseed.