复合型斜纹夜蛾核多角体病毒杀虫剂配比的筛选

Mixture Ratios Screening of Compound SpltNPV

为研制开发出高效、低毒的复合型斜纹夜蛾核多角体病毒配方,首先测定了斜纹夜蛾核多角体病毒(SpltNPV)、高效氯氰菊酯、甲氨基阿维菌素苯甲酸盐、溴虫腈4种药剂对4龄斜纹夜蛾幼虫的致死中浓度;然后采用共毒因子法,测定了SpltNPV和3种化学药剂组合的联合作用,结果发现SpltNPV+甲氨基阿维菌素苯甲酸盐组合的共毒因子为68.23,表现为增效作用;最后通过正交通用旋转回归设计法,获得了SpltNPV和甲氨基阿维菌素苯甲酸盐的最佳质量配比为1∶1。

In order to development Spodoptera litura nucleopolyhedrovirus（SpltNPV） compound formulation with efficient and low toxicity, the median lethal conccntration（LC50） against 4th-instar Spodoptera litura of SpltNPV, beta-cypermethrin, emamectin benzoate and chlorfenapyr were testes with bioassay method. Then with co-toxicity factor method, the associative actions of SpltNPV and other three chemical pesticides were studied. The results showed that the co-toxicity factor of SpltNPV and emamectin benzoate was the highest with 68.23. Last the optimal mixture ratios were got 1：1 in combination of SpltNPV and emamectin benzoate with quadratic regression rotation composite design method.