Evaluate the Expression of uPA,PAI-1 in Human Gastric Cancer and its Correlation with the Angiogenesis by the Application of Tissue Microarray

OBJECTIVE To investigate the expression of urokinase-type plasminogen （uPA）, its inhibitor-1 （PAI-1） mRNA and its protein in human gastric cancer and to find out the relationship among the tumor differentiation, angiogenesis, and other clinical pathologic factors. METHODS In situ hybridization （ISH） was used to get the uPA, PAI-lmRNA in 110 cases with human gastric cancer in 2-tissue microarray （TMA）. Immunohistochemical staining （S-P method） for uPA, PAI-1 protein and CD34 were performed in the 110 cases in 2 TMA. RESULTS The expression of the uPA, PAI-lmRNA and their protein happened in the cytoplasm of gastric cancer cells were induced by the poor differentiation of the GC, and the expression of uPA had an increasing trend while the expression of the PAI-1 had a decreasing trend. The microvessel density （MVD） had a positive correlation with the clinical stages and the significant relationship with the lymph node metastasis （P 〈 0.05）. The MVD in uPA positive group was significantly higher than those in uPA negative group （P 〈 0.05）. The expression of PAI-1 has no correlation neither with the clinical stages nor the lymph node metastasis. CONCLUSION The uPA play an important role in invasion and metastasis of GC through promoting angiogenesis. Interdicting the secretion and function of the uPA may allow the target therapy against the tumor invasion. As a new high-throughput technology, the tissue microarray is a valuable way to be used in clinical treatment.

OBJECTIVE To investigate the expression of urokinase-typeplasminogen (uPA), its inhibitor-1 (PAI-1) mRNA and its proteinin human gastric cancer and to find out the relationship among thetumor differentiation, angiogenesis, and other clinical pathologicfactors.METHODS In situ hybridization (ISH) was used to get the uPA,PAI-1mRNA in 110 cases with human gastric cancer in 2-tissuemicroarray (TMA). Immunohistochemical staining (S-P method)for uPA, PAI-1 protein and CD34 were performed in the 110 casesin 2 TMA.RESULTS The expression of the uPA, PAI-1mRNA and theirprotein happened in the cytoplasm of gastric cancer cells wereinduced by the poor differentiation of the GC, and the expressionof uPA had an increasing trend while the expression of the PAI-1had a decreasing trend. The microvessel density (MVD) had apositive correlation with the clinical stages and the significantrelationship with the lymph node metastasis (P < 0.05). The MVDin uPA positive group was significantly higher than those inuPA negative group (P < 0.05). The expression of PAI-1 has nocorrelation neither with the clinical stages nor the lymph nodemetastasis.CONCLUSION The uPA play an important role in invasion andmetastasis of GC through promoting angiogenesis. Interdictingthe secretion and function of the uPA may allow the target therapyagainst the tumor invasion. As a new high-throughput technology,the tissue microarray is a valuable way to be used in clinicaltreatment.