酶法提取分离甘薯渣可溶性膳食纤维的研究

Study on the Enzyme Based Extraction of Soluble Dietary Fiber from Sweet Potato Dregs

目的:提高甘薯渣中可溶性膳食纤维含量。方法:以干燥、粉碎的甘薯渣为原料,利用纤维素酶将原料中不可溶性膳食纤维转化为可溶性膳食纤维,同时用淀粉酶,糖化酶和碳酸钠去掉杂质,酒精沉淀,从而获得可溶性膳食纤维。结果:在单因素基础上通过正交实验确定最佳酶法提取可溶性膳食纤维条件为淀粉酶100μl,纤维素酶90μl,pH为5.6,酶作用时间为3h,反应温度为40℃。国标法提取的甘薯渣中可溶性膳食纤维含量为10.06%,正交实验优化提取条件后得到的甘薯渣可溶性膳食纤维含量为25.52%,提高了15.46%。结论:甘薯渣可溶性膳食纤维含量受纤维素酶,淀粉酶,酶提取时间,酶反应pH,反应温度等提取工艺影响。

Objective： To increase the yield of soluble dietary fiber （SDF） in sweet potato dregs. Method： The dried and smashed sweet potato dregs were used as the raw materials. First, the insoluble dietary fiber （IDF） was converted to SDF by cellulase in the materi- als. And the impurity in the sample was removed by treating with amylase, diastatic enzyme and Na2CO3 and then precipitated by adding ~ O ethanol. The precipitate was collected and the soluble dietary fiber （SDF） was obtained. Result： The optimized conditions to extract the SDF, explored through singer factors and orthogonal experiment, were 100 μl of amylase, 90μl of cellulase, reaction time of 3 h, temperature of 40℃, and pH of 5.6. The contents of the SDF in sweet potato dregs could be increased from 10.06% to 25.52% compared with the enzyme-gravimetric method, which raised by 15.46% .Conclusion： The yield of SDF in sweet potato dregs is affected by multi- ple factors, such as the quantity of amylase or cellulase, the reaction time, the pH value and reaction temperature.