胃肠道癌患者外周血淋巴细胞P16、cyclinD1异常表达及其临床意义

Clinical significance on expression of P16 and cyclinD1 in blood lymphocyte in patients with gastrointestinal cancer

目的:分析胃肠道癌患者外周血淋巴细胞p16、cyclin Dl表达的临床意义。方法:取43例健康人为对照组、病例组为73例经病理确诊的胃肠道癌患者。用流式细胞仪检测其外周血淋巴细胞p16和cyclin Dl的表达,用mann-whitney U检验分析病例组及对照组外周血淋巴细胞p16、cyclin Dl表达的差异性及外周血淋巴细胞p16、cyclin Dl在胃肠道癌患者不同分期中表达的差异,并绘制ROC曲线,确定临界值,计算p16、cyclin Dl的特异度和灵敏度。结果:胃肠道癌患者外周血淋巴细胞P16及cyclin D1表达水平均明显高于对照组,两组比较差异有显著性(P<0.001);绘制ROC曲线,复发临界值:P16为3.66;cyclin D1为3.84。灵敏度、特异度、准确率为:P16:80.8%、76.7%、79.3%;Cyclin D1:80.8%、56.8%、71.6%;两指标联合检测并联试验灵敏度达90.4%,串联试验特异度达83.7%。而胃肠道癌患者外周血淋巴细胞P16及cyclin D1表达水平则与临床分期无关(P<0.001)。结论:胃肠道癌外周血淋巴细胞p16、cyclin Dl表达高于正常人群,二者联合检测对胃肠道癌的判断有较高的灵敏度和特异度。

Objeetive： to analyse the clinical significance on the expression of P16 and cyclinD1 in blood lymphocyte in patients with gastrointestinal cancer, Methods： Flow cytometry was used to detect the expression of the PI6 and cyclinD1 in blood lymphocyte in control group and case group. The expressions in the two groups was analysed by mann-whitney U test; The Roc curve was drawn to def- inite the curital value; and the sensitivityand specificity of the P16 and cyclinD1 was calculated. Results： The expressions of the P16 and cyclinD1 in peripheral blood lymphocyte were significantly different in the two groups （P〈0.001）. The expressions of the P16 and cy- clinD1 in the case group, being not related to clinical staging, were significantly higher than those in control group. There was significant difference between the two groups（P〈0.001）. Conclusions： The expressions of the PI6 and cyclinD1 in peripheral blood lymphocyte were higher than those in the normal population, and the combinative detection of the two index had higher sensitivity and specificity to diagnose gastrointestinal cancer.