摘要
目的建立高效液相色谱法(HPLC)研究非索非那定灌胃给药后非索非那定体内药动学特点。方法采用HPLC测定,色谱柱为DikmaTMC18(4.6mm×250mm,5μm),流动相:乙腈-0.5%磷酸二氢钾(pH3.8)-三乙胺(30∶70∶0.3),流速:1.2ml/min,检测波长:220nm,检测非索非那定大鼠灌胃后药代动力学参数。结果非索非那定的药动学模型为二室模型,其药动学参数为Ka=1.689h-1,Cmax=1.225μg/ml,Tmax=1.8h,MRT=5.013h,t1/2α=1.018h。结论高效液相色谱法研究非索非那定大鼠灌胃给药后体内药代动力学,方法预处理简单、专属性强,结果可靠。
Objective To develop a method for the determination of fexofenadine in rat plasma after a single oral administration. Methods The supernatant was injected directly on a DikmaTM C18 column （250mm × 4.6mm,51μm） with the mobile phase consisted of a mixture of acetonitrile-0.5% KH2PO4 buffer-triethylamine （ 30： 70：0.3 ） at a flow rate of 1.2mL/ min. The UV detection wavelength was 220nm. The pharmaeokinetie parameters of fexofenadine were recorded. Results After orally taken fexofenadine, the plasma concentration-time course in rats fitted well to 2-compartment model and with the following pharmaeokinetie parameters ：Ka = 1. 689 h^-1,Cmax=1. 225μg/ml, Tmax= 1.8h,MRT=5. 013h,t1/aα = 1. 018h. Conclusion The HPLC method to determine plasma concentration of fexofenadine and pharamacokinetie parameters in rats is feasible.
出处
《临床合理用药杂志》
2009年第13期4-5,共2页
Chinese Journal of Clinical Rational Drug Use
