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信号转导和转录激活因子3在子宫颈脱落细胞的表达及临床意义 被引量：6

Expression and clinical significance of STAT3 in exfoliated cervical cells
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摘要 目的:研究信号转导和转录激活因子3(STAT3)及下游基因Survivin在子宫颈脱落细胞的表达及意义。方法:用RT-PCR方法和Western-blotting方法检测25例子宫颈癌患者和50例正常人的宫颈脱落细胞中STAT3及其下游基因Survivin的表达。结果:子宫颈癌患者宫颈脱落细胞中STAT3基因高表达,包括转录水平增高及蛋白表达水平增高,与正常宫颈脱落细胞的差异有统计学意义(P<0.05);下游基因Survivin mRNA在宫颈癌脱落细胞中的阳性表达率为84%(21/25),与正常组相比差异有统计学意义(P<0.05)。结论:STAT3,Survivin基因在宫颈癌细胞中异常表达,刺激细胞持续异常增殖,与宫颈癌的发生密切相关。 Objective： To investigate the expression and significance of STAT3 and Survivin in exfoliated cervical cells. Methods：The quantitative reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting were used to test the expression levels of STAT3 and Survivin in exfoliated cervical cells of 25 cervical carcinoma and 50 normal cervix. Results ：STAT3 was highly expressed in exfoliated cervical epithelial cells of cervical carcinoma at protein and transcription level. There was a significant difference between the two groups （ P 〈 0.05）. The positive percentages of Survivin mRNA expression in cervical carcinoma exfoliated cells were 84% ,and there was remarkably discrepancy than normal cervical exfoliated cells （P 〈 0.05 ）. Conclution： Abnormal expressions of STAT3 and downstream Survivin in cervical carcinoma ceils stimulate tumor cells proliferation, and maybe involve in cell growth and proliferation during the procedure of oncogenesis.
作者 卫莹 周颖 李敏 彭程 冯定庆 吴大宝 肖卫华 凌斌
机构地区 安徽医科大学附属省立医院妇产科分子实验室安徽省分子医学重点实验室 中国科技大学生命科学学院
出处 《现代妇产科进展》 CSCD 北大核心 2009年第7期504-506,共3页 Progress in Obstetrics and Gynecology
基金 安徽省自然科学基金资助(No:090413117)
关键词 宫颈肿瘤 子宫颈 细胞学 信号转导和转录激活因子3 基因 Survivin Cervix neoplasms Cervix uteri Cytology Signal transducer and activator of transcription 3 Genes, Survivin
分类号 R730.231 [医药卫生—肿瘤]
  • 相关文献

参考文献11

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二级参考文献11

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共引文献35

同被引文献39

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引证文献6

二级引证文献8

现代妇产科进展
2009年 第7期

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