CD44V6与Ezrin在非小细胞肺癌中的表达及临床意义

Significance of CD44V6 and Ezrin expression in human non-small cell lung cancer

目的:探讨CD44V6与Ezrin在肺癌组织中的表达、关系及临床意义。方法:采用免疫组化二步法检测65例肺癌及15例正常肺组织标本中CD44V6与Ezrin的表达,结合肺癌的临床病理特点进行分析。结果:CD44V6在NSCLC中的阳性表达率69.23%,与临床分期、淋巴结转移成正相关,与病理类型、组织分化程度无关;而在肺良性病变中无阳性表达。肺癌组织中Ezrin的阳性表达率(72.3%)较正常肺组织(100%)有所降低,两者比较差异有统计学意义(P<0.05)。Ezrin表达下降与肺癌患者病理类型、组织分化程度无关,与有无区域淋巴结及远处转移有关。此外,肺癌组织Ezrin表达有从胞膜向胞浆移位的倾向。结论:CD44v6与Ezrin在肺癌的发生,发展及转移过程中发挥调控作用,可作为判断肺癌转移及预后的重要生物学指标。

Objective：To study the expression of CD44V6 and Ezrin in human non-small cell lung cancer （NSCLC）. Methods：CD44V6 and Ezrin were detected by two-step immunohistochemical staining technique in tumor tissues from 65 lung cancer cases and in normal lung tissues from 15 patients with benign disease and analyzed by the lung cancer clinicopathologic characteristics. Results：The positive rate of CD44V6 expression in NSCLC was 69.23%, and no difference was detected in the normal lung tissues.The up regulated of CD44V6 expression was significantly correlated with lymph node metastasis and distant metastasis, but not correlated with pathological type, the degree of differentiation of tissue and clinical stage.The positive incidence of Ezrin expression （72.3%）was significantly lower in lung cancer tissues than that in normal tissues （100%）（P〈0.05）,and the down-regulation of Ezrin expression was significantly correlated with lymph node metastasis and distant metastasis, but not correlated with pathological type, the degree of differentiation of tissue. Also, Ezrin expression in lung cancer tended to move from cell membrance to cell plasma. Conclusion： CD44V6 and Ezrin are involved in carcinogenesis. The levels of CD44V6 and Ezrin may be a sensitive predictor for metastasis and prognosis in NSCLC.