高迁移率族蛋白A2在子宫内膜癌中的表达及意义

Expression and significance of high mobility group protein A2 in endometrial carcinoma

目的:探讨高迁移率族蛋白A2(high mobility group protein A2,HMGA2)在子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及子宫内膜癌组织、正常子宫内膜组织中的表达及意义。方法:采用逆转录-多聚合酶链反应(RT-PCR)、免疫印迹法(Western Blot)检测HMGA2在体外培养的子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及21例子宫内膜癌组织标本和18例正常子宫内膜组织标本中的表达。结果:HMGA2-mRNA在子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa中均有表达;在I期、Ⅱ期子宫内膜癌患者中表达的阳性率分别为8.3%(1/12)、88.9%(8/9),HMGA2-mRNA表达的阳性率与手术-病理分期有关(P=0.000,P<0.05),在高分化腺癌、中低分化腺癌的组织标本中表达阳性率分别为66.7%(4/6)、33.3%(5/15),HMGA2-mRNA表达阳性率与子宫内膜癌细胞的分化程度无关(P=0.331,P>0.05);HMGA2-mRNA在18例正常子宫内膜组织标本中无一例表达;Western Blot的结果与RT-PCR的结果相符合。结论:HMGA2基因可能参与了子宫内膜癌的发生和发展,在子宫内膜癌细胞的增殖与侵袭中可能发挥重要作用。

Objective：To investigate the expression and significance of high mobility group protein A2 （HMGA2） in endometrial carcinoma cell lines AN3CA, ECC - 1, Ishikawa and tissue specimen of carcinomatous endometrium and normal endometrium. Methods ： The expressions of HMGA2 - mRNA and HMGA2 protein were detected in endometrial carcinoma cell lines AN3CA, ECC - 1, Ishikawa and tissue specimen from cacinomatous endometrium（ n = 21 ） and normal endometrium（ n = 18） using reverse tanscriptase -polymerase chain reaction（ RT -PCR） and Western blot analysis, respectively. Results： HMGA2 - mRNA expressed in all of the endometrial carcinoma cell lines AN3CA,ECC - 1 ,Ishikawa . The positive expression rate of HMGA2 - mRNA in stage Ⅰ and stage Ⅱ tumors were 8.3% （1/12） and 88.9% （8/9）, respectively. The positive expression rate of HMGA2 - mRNA was correlated with clinical stage of endometrial carcinoma（ P = 0. 000, P 〈 0. 05 ）. The positive expression rates of HMGA2 - mRNA in the highly,moderately and lowly differentiation tumors were 66.7% （4/6）and 33.3% （5/15） ,respectively. The positive expression rates of HMGA2 - mRNA showed an insignificant difference in differentiated feature of cancer ceils（ P =0.331 ,P 〉0. 05）. HMGA2 -mRNA was not detected in all of the tissue specimen from normal endometrium. Western blot analysis showed similar results with RT - PCR method. Conclusion： The expression of HMGA2 gene may be involved in the development and tumorigenesis of endometrial carcinoma and maybe play very important roles in cell proliferation and invasion of endometrial carcinoma.