HE4基因表达载体的构建及对卵巢癌细胞HO8910生长侵袭的影响

Effect of HE4 Overexpression on the Proliferation and Invasion Abilities of the Ovarian Cancer Cell Line HO8910

目的探讨卵巢癌基因HE4对卵巢癌细胞增殖及侵袭能力的影响。方法构建针对HE4基因的PcDNA3.1/HE4载体,脂质体法介导PcDNA3.1/HE4载体转染人浆液性卵巢癌细胞系HO8910,WESTERN-BLOT检测转染前后细胞内HE4蛋白的表达效果。用四甲基偶氮唑蓝(MTT)比色法、平板克隆形成实验观察HE4基因对细胞增殖能力的影响,经细胞粘附实验、体外侵袭力实验比较转染前后细胞侵袭能力的变化。结果Western-blot结果显示PcDNA3.1/HE4载体成功转染HO8910细胞并显著提高该细胞HE4基因的表达,细胞增殖能力较前无明显变化,侵袭能力明显下降。结论通过增强HE4基因表达,可降低其侵袭能力,有望成为卵巢癌基因治疗的新的候选基因。

Objective To examine the effect of HE4 gene overexpression on the proliferation and invasiveness of the ovarian cancer cell line HO8910. Method HE4 carrying PcDNA3.1 vector （PcDNA3.1/HE4） was constructed and transfected into a highly metastatic human ovarian cancer cell line HO8910 with Lipofectamine 2000. The protein expression was determined by Western-blot. MTT and colony formation assay were used for cell growth measurement. Tumour cell invasiveness and cell adhesiveness were also determined. Result Overexpression of HE4 was confirmed by Western-blot and the growth was not affected by HE4 overexpression. However, the tumour cell invasiveness was reduced. Conclusion Overexpression of HE4 can reduce the invasiveness of HO8910 ceils, and HE4 may be a candidate gene for the treatment of ovarian cancer.