脂蛋白和C反应蛋白对单核细胞趋化蛋白-1的影响

Effects of Lipoprotein and C-reactive Protein on the Expression of Monocyte Chemoattractant Protein-1 and C-C Chemokine Receptor 2

目的探讨单核细胞趋化蛋白-1(MCP-1)及其受体趋化因子受体2(CCR-2)经动脉粥样硬化相关因素刺激后表达量的变化情况。方法密度梯度离心法从健康成人外周血中分离获得单个核细胞,体外培养48h。给予氧化低密度脂蛋白(ox-LDL)、C反应蛋白(CRP)及高密度脂蛋白(HDL)进行干预。干预后用实时荧光定量RT-PCR技术检测单核细胞MCP-1mRNA和CCR2mRNA的含量,ELISA法检测MCP-1蛋白含量。结果ox-LDL+CRP组、ox-LDL、CRP处理组MCP-1和CCR2mRNA表达水平和MCP-1蛋白含量明显高于对照组(P<0.05)。而经HDL处理的单核细胞表达MCP-1和CCR2mRNA水平下调,MCP-1蛋白含量低于对照组(P<0.05)。结论在体外培养的条件下,CRP和ox-LDL可以促进MCP-1和CCR2mRNA表达,继而导致MCP-1含量增加,增强炎症反应的范围和强度。HDL可以有效的抑制MCP-1的表达。

Objective To explore the modulation effect of atherosclerosis related factors on the expressions of monocyte chemoattractant protein-1 and its recpetor C-C chemokine receptor 2 （CCR2）. Method Healthy human peripheral mononuelear blood cells （PBMCs） were collected from femoral vessel and isolated by density gradient centrifugation. After cultured for 48 h, the cells were treated with oxidized low density lipoprotein （ox-LDL）, C- reactive protein （CRP）or high density lipoprotein （HDL）. Real-time Q-PCR was performed to test the mRNA expression of MCP-1 and CCR2. Enzyme linked immunosorbent assay was utilized for measuring level of MCP-1 protein. Result Compared with the control, CRP and ox-LDL enhanced the mRNA expression of MCP-1 and CCR2 and level of MCP-1 protein in vitro （P〈0.05）. HDL attenuated the expression of both mRNA and the level of MCP-1 （P〈0.05）. Conclusion CRP and ox-LDL enhanced the mRNA expressions of MCP-1 and CCR2 in vitro, leading to the increase of MCP-1 protein level and extension of inflammatory response. HDL efficiently inhibited the expression of MCP-1.