焦炉工人苯并[a]芘暴露与外周血淋巴细胞DNA损伤的关系

The Correlation between the Exposure to Benzo (a) Pyrene and DNA Damage in Peripheral Lymphocytes in Coke Oven Workers

目的探讨焦炉逸散物(coke oven emissions,COE)中苯并[a]芘对焦炉作业工人外周血淋巴细胞DNA损伤关系,寻找职业性肺癌的早期筛检指标。方法分别选取炼焦和非炼焦工人进行流行病学调查,收集个人职业暴露、年龄、性别、吸烟等信息,同时采用单细胞凝胶电泳技术评价外周血淋巴细胞DNA的损伤程度。结果焦炉作业工人的DNA断裂程度随生产环境的不同而不同,其炉顶、炉侧组均明显高于对照组,差异有统计学意义(P<0.01);专业工龄10～年的工人DNA损伤程度远高于专业工龄0～年及5～年者(P<0.01)。结论单细胞凝胶电泳法能够快速、敏感地检测炼焦工人淋巴细胞的DNA损伤,提示该方法对检测环境致癌物和致突变物可能有较好的应用前景。

Objective To explore the correlation between the exposure to Benzo （a）Pyrene in coke oven evaporator and DNA damage in peripheral lymphocytes. Method Coke oven workers and non-coke oven workers were selected as target group and control group, respectively. Age, period of exposure, sex, smoking etc were analyzed epidemiologically. DNA damage in peripheral lymphocytes was also detected. Result For coke oven workers, the extent of lymphocyte DNA breakage differed from working spots and exposure time, those working on the top of oven and side of oven had more serious DNA breakage than control （P〈0.01）, and longer exposure showed more serious DNA breakage（P〈0.01 ）. Conclusion Exprosure to coke oven can cause DNA damage in perioheral lymphocytes and the degree of damage is positively correlated with top and side position of the oven and the exprosure time. The single cell gel electrophoresis is a rapid, sensitive and useful tool to detect the peripheral lymphocyte DNA damage for coke oven workers and can be used to detect the effects of other environmental carcinogens or dismutants.