深圳市龙岗区患者痰培养细菌菌谱和耐药性研究

Study on the Spectrum and Drug Resistance of Bacteria Isolated Form Sputum Culture of Patients in Longgang District of Shenzhen

目的研究深圳市龙岗区患者痰培养细菌菌谱和耐药性,为临床合理应用抗菌药物提供依据。方法对来自深圳市龙岗区4间医院2007年1月至2008年6月患者合格痰标本中培养的细菌药敏试验进行回顾性分析。结果727株细菌中,革兰阴性杆菌595株,占81.8%,以鲍曼不动杆菌、铜绿假单胞菌为主;革兰阳性球菌132株,占18.2%,以凝固酶阴性葡萄球菌、金黄色葡萄球菌为主,而且凝固酶阴性葡萄球菌株数超过金黄色葡萄球菌。药敏结果提示革兰阴性杆菌及革兰阳性球菌对相当多数的抗生素耐药率较高。结论深圳市龙岗区患者痰培养细菌耐药情况较严重,条件致病菌菌株数比例较高。应加强药物尤其是抗菌药物的使用管理,规范应用抗生素。

Objective To study the distribution and drug resistance of the bacteria isolated from sputum culture of patients in hospital in Longgang district of Shenzhen, and to provide evidence for the rational use of antibacterial agents in clinic. Method All qualified sputum specimens from inpatients between January 2007 and June 2008 were cultured for bacterial isolation. The isolated bacteria were identified and tested for the susceptibility to antibiotics. Result 727 bacteria were isolated,most（81.2%,595/727）of which were Gram negative bacillus;a few of which were Gram positive （18.2%, 132/727）. The 2 most common isolated Gram negative bacillus were Escherichia cob and Pseudornonas aeruginesa;tbe 2 most common isolating Gram positive bacillus were Staphylococcus aureus and Coagulase negative Staphylococcus. The result of drug sensitive test showed that there were high rates of drug resistance in most bacteria strains. Conclusion The rate of drug resistance of pathogenic bacteria to antibiotics is high. And the proportion of opportunistic pathogen bacteria increased apparently. Management should be further strengthened for rational use of antibiotic agents.