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功能型DNA重组修复蛋白质RecR的体内分布示踪 被引量：3

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摘要 RecR是参与大肠杆菌(E.coli)同源重组的蛋白质,在真核生物中存在功能保守性组分.通过构建recR-yfp融合基因及其表达载体,对大肠杆菌菌体内RecR蛋白的分布情况进行了活体观察.显微镜观察结果表明,RecR蛋白一般在菌体的拟核区起作用.通过对比紫外线敏感性,Re cR-YFP在重组质粒上提高了recR缺陷型大肠杆菌的抗紫外线能力,表明重组质粒表达的融合蛋白RecR-YFP具有生物活性,能够提高recR缺陷型大肠杆菌的存活率.
作者 邱洁芳 潘学峰
机构地区 北京理工大学生命科学与技术学院
出处 《自然科学进展》 北大核心 2009年第7期704-710,共7页
基金 北京理工大学自然科学基金项目资助(1060050320804)
关键词 RecR 黄色荧光蛋白DNA修复 同源重组 大肠杆菌
分类号 Q939.1 [生物学—微生物学] Q51 [生物学—生物化学]
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参考文献16

  • 1Kalluri SR. Mechanisms of disease: DNA repair defects and neurological desease. Nature, 2007, 3(4):162- 172.
  • 2Mahdi AA, Lloyd RG. The recR locus of Escherichia coli K-12: Molecular cloning, DNA sequencing and identification of the gene product. Nucleic Acids Res, 1989, 17(17) : 6781- 6794.
  • 3Yeung T, Mullin DA, Chen KS, et al. Sequence and expression of the Escherichia coli recR locus. J Bacteriol, 1990, 10:6042--6047.
  • 4邱洁芳,潘学峰.大肠杆菌细胞DNA复制、修复和重组途径的衔接[J].生物化学与生物物理进展,2008,35(7):751-756. 被引量：2
  • 5Stauffer ME, Chazin WJ. Structural mechanisms of DNA replication, repair and recombination. J Biol Chem, 2004, 279(30): 30915- 30918.
  • 6Phuong P, Savithri R, Roger W, et al. Roles of DNA polymerases V and II in SOS-induced error prone and erroFfree repair in Escherichla coll. Proc Natl Acad Sci USA, 2001, 98(15): 8350- 8354.
  • 7Masayoshi H, Jin I, Masatoshi Y, et al. Identification of the RecR toprim domain as the binding site for both ReeF and RecO. J Biol Chem, 2006, 281(27): 18549- 18559.
  • 8Umezu K, Chi NW, Kolodner RD. Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RccA protein and singlc-stranded DNA binding protein. Proc Natl Acad Sci USA, 1993, 90(9): 3875-3879.
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二级参考文献30

  • 1Xu Y, Grindley N D F, Joyce C M. Coordination between the polymerase and 5' nuclease components of DNA polymerase I of Escherichia coll. J Biol Chem, 2000, 275(27): 20949-20955
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  • 9Asai T, Kogoma T. The RecF pathway of homologous recombination can mediate the initiation of DNA damage-inducible replication of the Escherichia coli chromosome. J Bacteriol, 1994, 176(22): 7113-7114
  • 10Bork J M, Cox M M, lnman R B. The RecOR proteins modulate RecA protein function at 5' ends of single-stranded DNA. EMBO J, 2001, 20:7313-7322

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自然科学进展
2009年 第7期

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