大肠癌发生与器官特异性转移机制及干预的研究

The Effect of Intervention on Pathogenesis and Homing Metastasis and the Associated Mechanism in Colorectal Carcinoma

随着我国城市化进程加快，人群饮食、生活习惯等因素改变，大肠癌发病率逐年增加，严重威胁人民健康，有关大肠癌发病机理及相关防治策略的研究一直是国内外肿瘤防治研究中的热点问题。本项目利用免疫组化、原位杂交、TU—NEL、Western blot和PCR等技术从动物、细胞和人体水平多角度阐述大肠癌发生、发展和器官特异性转移相关分子机制，并提出了相应的防治措施。研究过程中首次提出大肠癌发生中存在以凋亡受抑制为特征的“细胞选择性增殖”现象，舒林酸可通过诱导细胞凋亡防治大肠癌，率先提出预防效果优于治疗效果及Fas／FasL系统参与了凋亡诱导机制的观点，

With the accelerating process of urbanization in China, people＇s diet, lifestyle factors changes, as a result, the incidence of colorectal cancer increases every year. It has become a serious threat to the health of the people. The pathogenesis of colorectal cancer, its control strategies and research for cancer prevention and treatment has become a hot spot worldwide. In this study, immunohistochemistry, in situ hybridization, TUNEL, Western blot and PCR techniques were performed in the animal model, cell lines and human cases of colorectal cancer to detect the molecular mechanisms of the carcinogenesis, development and organ-specific metastasis in colorectal cancer. The corresponding control measures were proposed subsequently. For the first time, we put forward that apoptosis was inhibited,