Alterations and Its Mechanisms of Wnt Signal Pathway in Human High-matastatatic Large Cell Lung Cancer Cell Line L9981 by Transfecting with Nm23-H1 Gene

Backgroud and Objective Tumor metastasis is not only the malignant marker and characteristics of lung cancer, but also the main cause of failure to cure and lose their life of the

Backgroud and Objective Tumor metastasis is not only the malignant marker and characteristics of lung cancer, but also the main cause of failure to cure and lose their life of the patients with lung cancer. It is a complex biological behavior that dealed with many factors, genes, signal pathways and processes. Therefor, exploration of the changes of cell signal transduction related to invasion and metastasis in lung cancer will not only illuminate the molecular mechanism of tumor invasion and metastasis,but also provide a new targeting molecule and route for blocking signal transduction and reversing metastatic phenotype of lung cancer. Now, it has been proved that low expression and hetero-deletion of tumor metastasis suppressor gene nm23-H1 was closely correlated with the high metastasis ability and poor prognosis of patient with lung cancer, nm23-H1 gene is a key and upstream regulative gene in the ＂lung cancer metastatic suppressive cascade＂. Transfection of wild type nm23-H1 cDNA into human high-metastatic large cell lung cancer cell, which has been proved that it had LOH ofnm23-H1 gene, can regulate the expression of metastatic relative genes and reverse the metastatic phenotype of lung cancer cell lines.