摘要
背景与目的细胞与生物医用材料之间的相互作用是影响其临床应用的重要因素之一,本研究旨在探讨三种缝线材料对人肺腺癌细胞A549增殖和细胞周期的影响。
方法将三种缝线材料分别与A549细胞直接培养72h后,采用MTT法测定各组吸光度,反映各组A549细胞的增殖情况,用流式细胞技术检测各组G1期细胞和S期细胞所占比例。
Background and objective The interaction of cell and medical biomaterial is one of the significant factors to affect clinical application of medical biomaterial. This research is to investigate three of suture lines how to affect the proliferation and cell cycle of Lung Adenocarcinoma cell A549.
Methods Three of suture lines are respectively cultivated with Lung Adenocarcinoma cell A549, after of 72 hours, to detect absorptance of each groups by MTF method in order to reflect the proliferation of Lung Adenocarcinoma cell A549 and to detect percentage of G1 period cell and S period cell of each of groups by flow cytometry.
出处
《中国肺癌杂志》
CAS
2009年第6期482-483,共2页
Chinese Journal of Lung Cancer
基金
国家自然科学基金面上项目(No.30872555)
云南省自然科学基金(No.2008ZC139M、2009CD)
云南省高层次人才培引工程(No.20080C014)资助~~
关键词
缝线材料
肺腺癌
细胞增殖
细胞周期
Suture line
Lung adenocarcinoma
Cell multiplica-tion
Cell cycle