Down-regulation and It's Molecular Biology of Nm-23H1 Gene Transfection on PKC Signal Path-way in Human High-metastatic Large Cell Lung Cancer Cell Line L9981

Backgroud and Objective At present, lung cancer has become the most frenquent malignant tumor in China and in the world which its mortality and morbidity is increasing fastest.

Backgroud and Objective At present, lung cancer has become the most frenquent malignant tumor in China and in the world which its mortality and morbidity is increasing fastest. It becomes NO.1 in leading malignant tumors, which is harmful to human health and life, also is the first killer in all cancers. Tumor metastasis is not only the malignant marker and charateristics of lung cancer, but also the key cause of failure to cure and high mortality in lung cancer. Studying and understanding the molecular mechanism of its invasion and metastasis provide a new targeting molecules and pathway for retro-transduction of the signal pathway. Our research group guided by Dr. Qinghua Zhou, keep on studying in this area for a long time. we have first established three cell lines in the world： L9981, L9981-pLXSN and L9981- nm23-H1. An expression plasmid of pLXSN-nm23-HI-EGFP with EGFP and wild nm23-H1 gene has been successfully constructed and transfected into the L9981 cell line. We have proved that low expression and hetero-deletion of tumor metastasis suppress gene nm23-H1 to be correlated with the high metastasis ability and poor prognosis in patient with lung cancer.