电麻处理对低温贮运鲫生理生化的影响

Effects of electric stunning on physiological and biochemical characteristics of crucian before stored at low-temperature

研究电麻处理对低温贮运鲫生理生化的影响,确定适宜的电麻电压和时间。对鲫（Carassius auratus）用不同电压（0,10,15,20,25,30 V）电麻5 min及用20 V电压电麻不同时间（0,2,4,6,8,10 min）后低温贮运,测定其存活率、血液生化指标、氨氮排泄量。结果表明,电麻处理可降低鲫对暂养水温急剧变化的应激反应,20 V电麻4-6 min时存活率最高,电麻组鲫的皮质醇、血糖、乳酸脱氢酶、谷草转氨酶活性及氨氮排泄量均低于对照组,其中用20 V电麻6 min时鱼体内血液指标活性最低,低温贮运保活效果最好。

The effects of electric stunning on physiological and biochemical characteristics of crucian （ Carassius auratus） before stored at low-temperature were studied, in order to provide foundation for the optimum voltage and time of electric stunning. The crucian were stunned using 0,10,15,20,25 and 30 V for 5 min or 20 V for 0,2,4,6,8 and 10 min,then they were stored at low-temperature. The survival rate,blood biochemical indexes and ammonia-N excretion were determined. The results showed that electric stunning could depress the stress response of crucian for the rapid changes of water temperature. The survival rate was the highest when electric stunned using 20 V for 4 - 6 rain. The contents of cortioal, glucose,lactate dehydrogenase, aspartate aminotransferase and ammonia-N excretion of electrical stunning groups were lower than those of the control group. The blood biochemical indexes were the lowest when electric stunned using 20 V for 6 min. The effect was the best when they were stunned using 20 V for 6 rain before stored at low-temperature.