酗酒对重型脑外伤患者脑内脱辅基脂蛋白E水平的影响及意义

Expression of apolipoprotein E in alcoholic patients with severe traumatic brain injury

目的探讨酗酒对重型脑外伤患者脑内脱辅基脂蛋白E（apoE）合成的影响及意义。方法采用RT—PCR法检测17例酗酒患者（研究组）和22例非酗酒患者（对照组）挫伤脑组织apoEmRNA的表达。并采用ELISA法测定两组患者脑外伤后不同时期脑脊液apoE含量。结果研究组脑组织apoEmRNA的表达水平及脑脊液apoE的含量较对照组降低（P〈0．05）。结论长期酗酒可使脑外伤后脑内apoEmRNA的表达水平降低，从而抑止脑内apoE的合成和分泌。

Objective To investigate the expression of apolipoprotein E in alcoholic patients with severe traumatic brain injury. Methods The study enrolled 17 alcoholic patients and 22 non-alcoholic patients with severe traumatic brain injury. The apolipoprotein E mRNA of contused brain tissue and apolipoprotein E protein in CSF were detected by reverse transcription-polymerase chain reaction （RT-PCR） and enzyme linked immunosorbent assay （ELISA） respectively. Results The expression of apolipoprotein E mRNA in brain tissue and apolipoprotein E protein in CSF were lower in alcoholic patients than those in non-alcoholic patients （both P〈0.05）, Conclusion Alcohol drinking may inhibit the synthesis of apolipoprotein E in brains after traumatic brain injury.