参附注射液预处理对大鼠心肌缺血再灌注损伤时心肌能量代谢的影响

Effect of Shenfu Injection on Myocardial Energy Metabolism Following Acute Myocardial Ischemic Reperfusion in Rats

目的探讨参附注射液预处理对大鼠心肌缺血再灌注损伤心肌的能量代谢的影响及其可能机制。方法健康SD大鼠36只随机分为空白对照组(C组)、缺血再灌注组(IR组)、参附注射液预处理组(SF组),每组12只。采用钳闭左冠状动脉前降支30min,再灌注2h复制心肌缺血再灌注模型。利用高效液相色谱仪测定大鼠心肌组织中的高能磷酸化合物ATP、ADP及AMP的含量,硫代巴比妥酸比色法测定丙二醛(MDA)含量,黄嘌呤氧化酶法测定过氧化物歧化酶(SOD)活性。结果IR组心肌组织中ATP及AMP含量均明显低于C组和SF组,同时ADP亦低于SF组。SF组大鼠心肌组织内的ATP含量低于C组,但ADP及AMP含量均明显高于C组。IR组MDA含量显著高于C组和SF组;但C组和SF组SOD活性显著高于IR组。结论参附注射液预处理可通过提高心肌组织中高能磷酸化合物的含量,降低MDA含量和保护SOD活性,对心肌缺血再灌注损伤产生保护作用。

Objective：To study the effect and the mechanism of Shenfu Injection on myocardial energy metabolism following acute myocardial ischemic reperfusion. Methods： 36 SD rats were randomly divided into three groups each consisting of 12 rats： sham group（C group）, ischemic reperfusion group（IR group）, Shenfu Injection pretreatment group（SF group）. Ischemic reperfusion models were made by ligated left coronary artery. The HPLC was used to detect the contents of ATP, ADP and AMP. MDA was measured by thiobarbiturate colorimetry, and SOD activity was detected by xanthine oxidase technique. Results：The contents of ATP and AMP in IR group were significantly lower than those in C and SF group. The concentration of ADP was lower than SF group. In the SF group, the content of ATP was lower, but ADP and AMP were higher than those of C group. The content of MDA in IR group was higher significantly than that of C group and SF group. However, the activity of SOD was lower in IR than that in C and SF group. Conclusion： Shenfu Injection can protect ischemic reperfusion injury by raising the content of high energy phosphate and SOD activity in myocardium, meanwhile decreasing the content of MDA.