摘要
利用银染S-SAP技术对苹果富士及其14个芽变品种进行研究,结果发现,筛选出的6对引物中,L12/E7能够将所有富士芽变品种同富士区分开来,其余5对引物能够在芽变品种中区分秋富5号、烟富1、盛放富1和长富1号.由于S-SAP多态性主要由基因组内逆转座子的转座引起,因此富士苹果芽变品种的产生可能与逆转座子的插入有关.
＇Fuji＇ apple and its 14 bud mutants were identified with Silver staining S-SAP （Sequence-specific amplification polymorphism）. In six primer pairs screened from 44 primer combinations,L12/E7 could discriminate all the bud mutants from maternal ＇Fuji＇ successfully, other 5 primer pairs could discriminate ＇Akifu 5＇＇Yanfu 1＇ ＇Shengfangfu 1＇ and ＇Nagafu 1 ＇ mutants. The polymorphism in S-SAP mainly comes from the transposition of retrotransposon. The results suggested that the bud mutants generated in ＇Fuji＇ appeared to bederived from retrotransposon insertion.
出处
《西北植物学报》
CAS
CSCD
北大核心
2009年第6期1111-1115,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金资助项目(30200190,30740034)
关键词
富士
S-SAP
芽变
鉴定
Fuji
S-SAP
bud sports
identification
