生草乌及清蒸草乌遗传毒性检测分析

Research on genotoxicity of Radix Aconiti Kusnezoffii and Radix Aconiti Kusnezoffii steamed

目的探讨生草乌及清蒸草乌的遗传毒性。方法应用鼠伤寒沙门菌体外回复突变试验(Ames)和彗星实验(Cometassay)对生草乌及清蒸草乌的遗传毒性进行研究。结果生草乌Ames试验中TA97、TA100、TA102菌株无论代谢活化或非代谢活化,皆为阳性,而TA98菌株则为阴性;清蒸草乌Ames试验为阴性。彗星实验在生草乌1.25,2.5,5.0mg/mL浓度下,尾长分别为(16.55±7.81),(23.68±8.32),(30.18±11.35)px,尾部DNA含量分别为(13.65±6.21),(20.55±8.55),(28.72±13.39)px2,与阴性对照组尾长(8.08±2.59)px、尾部DNA含量(6.33±4.16)px2比较,差异有统计学意义(P<0.01),且呈剂量-效应关系;而清蒸草乌各剂量组与阴性对照组比较差异无统计学意义。结论生草乌具有一定的遗传毒性,而清蒸草乌无遗传毒性,清蒸起到了较好的减毒效果。

Objective To study the genotoxicity of Radix Aconiti Kusnezoffii and Radix Aconiti Kusnezoffii steamed. Methods The Ames test and Comet assay were used to detect the genotoxicity of Radix Aconiti Kusnezoffii and Radix Aconiti Kusnezoffii steamed. Results With and without S9 activation, there was positive result of Radix Aconiti Kusnezoffii and negative result of Radix Aconiti Kusnezoffii steamed. At the dose of 1.25,2.5,5.0 mg/mL Radix Aconiti Kusnezoffii, the tail length were 16. 55 ± 7.81,23. 68 ±8. 32,30. 18 ±11.35px and the tail DNA% were 13.65 ±6. 21,20. 55 ± 8.55, 28.72 ±13.39 px2. Compared with the control group, there were significant differences（P 〈 0. 01 ）. There was no significant difference between the negative control and the treated groups of Radix Aconiti Kusnezoffii steamed（P 〉 0. 05）. Conclusion Radix Aconiti Kusnezoffii could cause genotoxicity, while Radix Aconiti Kusnezoffii steamed could not cause genotoxicity. Attenuation of steaming on Radix Aconiti Kusnezoffii is significant.