ADAM33基因多态性与新疆汉族哮喘的相关性研究

Association between single nucleotide polymorphisms of rs511898 of ADAM33 gene and bronchia asthma in Xinjiang Han population

目的:探讨新疆汉族支气管哮喘与ADAM33基因rs511898位点多态性的关系。方法:采用聚合酶链反应和限制片段长度多态性(PCR-RFLP)方法对63例新疆汉族支气管哮喘患者病例组和70例无血缘关系的健康对照组进行ADAM33基因rs511898位点单核苷酸多态性(SNP)分析。结果:ADAM33基因rs511898位点病例组和对照组中基因型分布均符合Hardy-Weinberg平衡定律;病例组rs511898位点的基因型频数分布:GA为31(34.6),GG为28(24.2),AA为4(4.3),对照组该位点基因型频数分布为GA为42(38.4),GG为23(26.8),AA为5(4.7),2组间基因型频数分布无统计学意义(P>0.05)。G、A等位基因频数分布在2组间比较差异无统计学意义(P>0.05)。结论:ADAM33基因rs511898位点等位基因突变与新疆汉族支气管哮喘的发生没有明显的关联性。

Objective： To investigate the genetic association of bronchial asthma with ADAM33 gene polymorphism in Xinjiang Han population. Methods： PCR-RFLP were used to examine the single nucleotide polymorphism （SNP） of ADAM33 gene in 63 with bronchial asthma （case group） and 70 healthy individuals （control group）. Results： The genotype distributions on rs511898 of ADAM33 gene in case group and control group did not deviate from Hardy-Weinberg equilibrium. The frequency distribution of rs511898 in case group as follows： 31 were GA （34; 6）, 28 were GG （24.2）, 4 were AA （4.3） ; the frequency distributions on rs511898 in control group as follows： 42 were GA （38.4）, 23 was GG （26.8）, 5 were AA （4.7）. And there was no significant difference of frequency distribution of three genotypes between case group and control group （P 〉0.05） and there was no significant difference of frequency distribution of allele A and G between two groups either （P〉0.05）. Conclusion： The mutant allele of rs511898 of ADAM33 gene may not be associated with bronchial asthma in Xinjiang Han population.