肝X受体在阻塞性睡眠呼吸暂停低通气综合征的表达及意义

The expression and meaning of liver X receptor in obstructive sleep apnea-hypopnea syndrome patients

目的探讨肝X受体(LXR)及其表达基因胆固醇脂转运蛋白(CETP)、环氧化酶-2(COX2)在阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者中的作用及意义。方法收集2007年8月至2008年8月兰州大学第一医院43例OSAHS患者和45名正常人晨血,经分离培养后得到巨噬细胞,应用逆转录-聚合酶链反应(RT-PCR)凝胶电泳检测法测相关基因表达。结果OSAHS患者LXR、COX2、CETP表达高于对照组(P<0.05);OSAHS患者LXR、COX2、CETP与其呼吸暂停低通气指数(AHI)、最低血氧饱和度(LSaO2)均具有相关性;OSAHS患者LXR、CETP的表达在轻中重3组间存在增强趋势(P<0.05),COX2的表达轻中重3组间没有明显的增强趋势(P>0.05),但在重度与轻度组间差异有统计学意义(P<0.05)。结论OSAHS患者LXR表达显著增高,对CETP、COX2起到调节作用。

Objective To study the liver X receptor （LXR） and the gene expression of cholesterol ester transfer protein （ CETP）, cyclooxygenase-2 （COX2） in obstructive sleep apnea hypopnea syndrome （OSAHS） patients. Methods Macrophages were separated from the blood drawn in 30 cases of OSAHS patients and 30 healthy control subjects in the morning,RT-PCR gel eletrophoresis was used to determine the patterns of gene expression. Results OSAHS patients had higher LXR, COX2, CETP expression（ P 〈 0. 05 ） ; LXR, COX2, CETP were related to AHI, LSaO2 % ; OSAHS patients with higher AHI had higher LXR,CETP expression（P 〈 0. 05） ,COX2 gene expression was different between mild and severe （P 〈 0. 05）. Conclusion OSAHS patients LXR significantly increased the expression of COX2 and CETP and play a regulating role.