聚合酶链反应技术检测生殖支原体感染

Study of Mycoplasma genitalium by polymerase chain reaction technique

建立多重聚合酶链反应（ＰＣＲ）技术检测生殖支原体（Ｍｇ），设计Ｍｇ种属特异性引物及功能性结构蛋白基因引物，先进行标准株的特异性与敏感性试验，然后进行临床标本的ＰＣＲ扩增反应。分泌物标本用ＮＰ４０裂解液一步法制备模板ＤＮＡ。三对引物有良好的特异性，对Ｍｇ标准株模板敏感性可达１０－６μｇ。临床标本检测，广东地区性病门诊２６１例的ＭｇＤＮＡ阳性率明显高于上海、常州及南京地区（χ２＝１６．１，Ｐ＜０．０１），后三者的感染差异无显著性（χ２＝０．０６，Ｐ＞０．０５）。性病门诊患者５１１例的Ｍｇ总感染率与５６例正常对照组相比有高度显著性差异（χ２＝７．９，Ｐ＜０．０１）。

The technique of polymerase chain reaction(PCR) was developed to detect a new kind of mycoplasma genitalium(Mg). Three sets of primers were used here. One was special for genera of Mycoplasmas- 16S rRNA and the others were specific to the protein adhesion of Mg— Mg Pa. The results showed that specificity of 3 sets of primers was good and sensitivity to Mg DNA template was up to 10- 6μ g. The Mg infection rate in GuangDong was higher than that of other areas (χ 2=16.6, P< 0.01) and total Mg infection rate in STD clinics was also higher than that of normal group(χ 2=7.9, P< 0.01).