摘要
目的探讨成人型多囊肾病(APKD)症状前诊断。方法应用PCR技术扩增PKD1位点两侧的微卫星SM7、CW2、AC2.5和KG8为遗传标记,对广西柳州地区10个APKD家系97名成员(包括29名APKD患者)进行基因连锁分析。结果被检测家系疾病均与PKD1连锁,并确定7名28岁以下无症状且B超未发现肾脏囊肿的个体为PKD1基因携带者,从而作出症状前诊断。
Objective To study presymptomatic gene diagnosis in adult polycystic kidney disease (APKD). Methods Linkage analysis and haplotype characterization were carried out in 10 unrelated Chinese APKD families from Guangxi region, including 97 individuals and 29 affected members. Members of these families were ultrasonographically examined. Linkage to PKD1 was investigated in all families with four microsatellite markers(SM7, CW2, AC2.5, and KG8). These flanking markers were amplified by PCR.Results The families were fully informative for gene diagnosis and no evidence of genetic heterogeneity was found. DNA analysis predicted 7 asymptomatic individuals(by 28 years old) as being affected, although they all presented negative ultrasonographs.Conclusion Linkage analysis is still an important way in APKD presymptomatic gene diagnosis.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1998年第4期218-220,共3页
Chinese Journal of Medical Genetics
基金
高校博士学科点基金