中重度哮喘急性发作患儿血中EAN-78的表达及甲基强的松龙对其表达的影响

Expressions of epithelial cell-derived neutrophil-activating protein-78 in moderate and severe acute asthma children and the influence of methylprednisolone

目的:观察中重度哮喘急性发作患儿血中内皮源性中性粒细胞激活肽-78(EAN-78)的表达水平及甲基强的松龙(甲强龙)对其的影响。方法:选择中重度哮喘急性发作患儿为观察对象,分为甲强龙干预前、后组,小儿外科斜疝患儿(无哮喘病史)为对照组,留取外周血标本检测中性粒细胞(PMN)数目,并采用流式细胞术检测EAN-78的含量。结果:观察组血ENA-78水平明显高于对照组(P<0.01),其中干预前组血ENA-78水平又明显高于干预后组(P<0.01);观察组外周血PMN均分别较对照组明显增高,差异有统计学意义(P<0.01),但干预前、后两组外周血PMN计数比较差异无统计学意义(P>0.05);甲强龙干预前组血ENA-78的表达与PMN数目呈显著正相关(n=32,r=0.865,P<0.01)。结论:中重度哮喘急性发作时患儿血中ENA-78的表达及PMN数量是升高的,它们可能共同参与了哮喘发病的炎症过程;甲强龙部分通过抑制ENA-78的表达来减轻气道炎症。

AIM： To observe the expressions of epithelial cell-derived neutrophil-activating protein-78 in acute asthma of severe cases and the influence of methylprednisolone. METHODS： Children with moderate and severe acute asthma were divided into urbason preinterfere group, post-interfere group, and the pediatric surgery indirect hernia children as control group （without asthma case history）, the numbers of heterophil granulocyte （PMN） in peripheral blood preparation were detected, and the expressions of EAN-78 protein were measured by flow eytometry. RESULTS： Compared with the control group, the levels of ENA-78 protein in asthma group were higher（ P 〈 0.01 ）. Compared with post-interfere group, the levels of ENA-78 protein in pre-interfere group were significantly higher （P 〈 0.01 ）. Compare with the eontrol group, the numbers of peripheral blood PMN in pre-interfere and post-interfere group were increased, there were significant differences（ P 〈 0.01 ）, and there were no significant differences about the numbers of PMN between pre-interfere and post-interfere group （P 〉 0.05 ）. There was a positive correlation between the levels of ENA-78 protein and the numbers of PMN by methylprednisolone （ n = 32, r = 0.865, P 〈 0.01）. CON- CLUSION： The levels of ENA-78 protein and the numbers of PMN in blood are increased in moderate and severe acute asthma children. They may be involved in the inflammation process of asthma pathogenesis. Methylprednisolone can relieve the airway inflammation by inhibiting the expressions of ENA-78 protein.